Download Advances in Chromatography: Volume 41 by Phyllis R. Brown, Eli Grushka PDF

By Phyllis R. Brown, Eli Grushka

An updated reference, supplying an leading edge standpoint on breakthroughs in separation technological know-how, akin to reverse-phase HPLC, advances in hyphenation, and linear dependence of relative retention values at the nature of the provider fuel and ordinary column strain in gas-liquid chromatography.

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Provided the size of the pores is large compared to the thickness of the electric double layer, the linear velocity is virtually independent of the mean channel diameter and directly proportional to the local electric field strength. This property of the electroosmotic flow makes it possible to employ separation columns in CEC that cannot be used (because of their high streaming resistance) in pressuredriven LC. Knox and Grant [13] examined the experimental conditions under which veo is independent of the mean channel diameter.

The upper limit of allowable capillary inner diameter is a function of the applied field strength, the specific conductance of the mobile phase, and the diffusion coefficients of the solutes. Knox [44] calculated for capillary electroseparation methods the boundary conditions under which the plate-height contribution of the thermal effects is less than 10% the plate-height contribution from axial diffusion. It is interesting to note that, according to Knox, with parameters commonly given in CEC (concentration of buffering salt in mobile phase ϭ 1 mmol/L3, applied field strength ϭ 100 kV/ m), the maximum allowed capillary inner diameter is 150 µm.

5 to 50 µm. In accordance with theory, the plate height decreases with decreasing particle size. 3 were observed. On a 240-mm-long column, about 120,000 plates were generated (holdup time ca. 4 min, ca. 500,000 plates/m) [41]. The authors 16 / Pyell state that in order to realize the observed linear velocity under pressure, about 1900 bar is required. 5-µm nonporous octadecyl silica gel, Bailey and Yan [42] were able to separate a series of 14 nitroaromatic and nitramine explosive compounds in under 7 min (see Fig.

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